Insertion junction: CLIP2.010044_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.010044
Chromosome:chromosome 6
Location:2339605
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g267700 SPPA1-2,SPP1B,SPP2 Signal peptide peptidase; (1 of 1) PF00574//PF01343 - Clp protease (CLP_protease) // Peptidase family S49 (Peptidase_S49) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTACGGTCTGGTCGAGACATGGTAAGCGCATGTATCCATGGGCTCACA
Internal bar code:GGTGTAGATAATACTATCTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1546
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACCGCTCACTTGTACTT
Suggested primer 2:AAACCATCGGACCGTTACCC