Insertion junction: CLIP2.010044_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.010044
Chromosome:chromosome 7
Location:6139072
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTTATTATATGTCACATGATAACATTTCGGCAGTCTAGGGCATTGGAC
Internal bar code:TTTGGGCTGTACTTTGGTATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2166
LEAP-Seq percent confirming:57.4074
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAATACCATGCCTCAAG
Suggested primer 2:AGCGCTCGTATCTTCCTTCG