| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010047 |
| Chromosome: | chromosome 11 |
| Location: | 222260 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467554 | (1 of 15) PF01694 - Rhomboid family (Rhomboid) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGGTGTCTCGCTTGGCTAGTCTGCTATAGGGGTAGGTCAGGAGGCA |
| Internal bar code: | CCAACTGGTGTTCGTTTCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1807 |
| LEAP-Seq percent confirming: | 97.6471 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCCTTCACGACGGAGAT |
| Suggested primer 2: | ACACATGGACGCAAAGCCTA |