| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010049 |
| Chromosome: | chromosome 10 |
| Location: | 1506266 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428678 | GID1,MNMG1,MNMG | (1 of 1) K03495 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme (gidA, mnmG, MTO1); tRNA uridine 5-carboxymethylaminomethyl modification enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTTATGGTTGTGTTCGGGTCAGCGGGTGCTCGATTTCACATGCAATC |
| Internal bar code: | TGTCGTTGAGTAGTTTGCTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1475 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACAGCCTCACAAACACG |
| Suggested primer 2: | TGTGCATCTTCGCCTTTCCT |