| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010058 |
| Chromosome: | chromosome 8 |
| Location: | 2852497 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374000 | (1 of 1) PF10444 - Nbl1 / Borealin N terminal (Nbl1_Borealin_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTTGTTGGTGTTTGGAAGAGCAGGGTGCAGGGGTGTGGGCATGCGGG |
| Internal bar code: | AGGTTAGCCTGAGGAATGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1062 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCCTGTAACACCCCAA |
| Suggested primer 2: | GTCGTCCACTGTGAACGTCT |