| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.010061 |
| Chromosome: | chromosome 1 |
| Location: | 4995539 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034600 | (1 of 1) PTHR22847:SF430 - MZB10.11 PROTEIN-RELATED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTTGCAGTTTGGCTCTGAGGCTTACCCAGCCCGCCCCTTCGGTGCGA |
| Internal bar code: | TAGTTATGGCAACTACCATGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2171 |
| LEAP-Seq percent confirming: | 70.7317 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGTGGTAATGGTGCAGA |
| Suggested primer 2: | GTTGTGCAGTAGAGGGCTGT |