| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.010084 |
| Chromosome: | chromosome 3 |
| Location: | 6075322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190250 | SUB6 | (1 of 5) PTHR10795//PTHR10795:SF157 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // MEMBRANE-BOUND TRANSCRIPTION FACTOR PEPTIDASE, SITE 1; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGGCTACCAGCCCAGCAGCGCCGGTGACGTGCGACTGTTTGATCTGG |
| Internal bar code: | GACTGTATATTGCCTTAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3618 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTCACTGTACGACACCAA |
| Suggested primer 2: | GATTCCTTCCTGTGGCGGAA |