Insertion junction: CLIP2.010109_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.010109
Chromosome:chromosome 4
Location:2022324
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g219150 (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAGAAATGTGAGGCCCCAATCATCACTACTGCCACTGCTACTGCTGTC
Internal bar code:CCTTTCCCTCCGGTTAGTAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2187
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGAATGTGATGGTGGAGC
Suggested primer 2:AACTGCATCTGGGTCGAGAC