| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010141 |
| Chromosome: | chromosome 9 |
| Location: | 5057364 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406000 | BPL1 | Biotin-protein ligase; (1 of 1) 6.3.4.11//6.3.4.15 - Biotin--[methylcrotonoyl-CoA-carboxylase] ligase / Biotin--[methylcrotonoyl-CoA-carboxylase] synthetase // Biotin--[acetyl-CoA-carboxylase] ligase / Biotin--protein ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACATCAGCATGTGCTCCGCTCGTTTTAGTTGGTTTTGGGTTAGTTTGG |
| Internal bar code: | GGTAACTCTTGGTTCTCAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1365 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGAATGGGGGTTAACGT |
| Suggested primer 2: | TGAACACATCCTCCATGGCC |