| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010145 |
| Chromosome: | chromosome 16 |
| Location: | 1772272 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654992 | (1 of 1) 2.1.1.225 - tRNA:m(4)X modification enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTCTAGGAAGTGCGGCTGGGCCTGCAAACACTTAAACGGGCGCAAGG |
| Internal bar code: | AGCCCACGAAGGCCTTCTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2395 |
| LEAP-Seq percent confirming: | 93.8775 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACACACAGTCTTCGCG |
| Suggested primer 2: | GTCTAGGATCGCCAGGAACG |