| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.010145 |
| Chromosome: | chromosome 16 |
| Location: | 1772283 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654992 | (1 of 1) 2.1.1.225 - tRNA:m(4)X modification enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAGAGGACGTGAATGCCGGGAGCGACGACGAGCCGAACCTGCCGCCG |
| Internal bar code: | CTTGCGTAGCAATAAAGTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1589 |
| LEAP-Seq percent confirming: | 90.2439 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTAGGATCGCCAGGAACG |
| Suggested primer 2: | CAACACACACAGTCTTCGCG |