Insertion junction: CLIP2.010152_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010152
Chromosome:chromosome 3
Location:7274394
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g207351 (1 of 2) PF10998 - Protein of unknown function (DUF2838) (DUF2838) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCATAGATCGCGGCTGCGACTCCGTGGCGTGGCGACGGCAGCCGCGCA
Internal bar code:AGGGCGCGATTACCCCGCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1770
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTCATGCAACAGCAACAGCA
Suggested primer 2:CCAGGAACTCCACCACATCC