Insertion junction: CLIP2.010156_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010156
Chromosome:chromosome 1
Location:3210212
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800065 (1 of 1) IPR001841//IPR025654 - Zinc finger, RING-type // Peroxisome biogenesis factor 10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAGTTGCACCACAGCCCGCGTCGCAAGCTGCCAAGGGATTAAACTGCT
Internal bar code:CCAACTGGCCGTTTGCCCTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:806
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGCCACTCTGATGAGGC
Suggested primer 2:TCGCAAACCAAGTCTCCTCC