Insertion junction: CLIP2.010168_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010168
Chromosome:chromosome 6
Location:918791
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256050 (1 of 19) 3.6.1.3 - Adenosinetriphosphatase / Triphosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAAAATGGGCGATCCTGGGGGACTTTGACTCCCTCCCGGAGTCGCTGC
Internal bar code:GCTGCGGAATGGGAATTTTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:499
LEAP-Seq percent confirming:52.381
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGCCGCGTGTTAGGTAAA
Suggested primer 2:AGTGTCGAACGGTGTCACTC