| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010171 |
| Chromosome: | chromosome 3 |
| Location: | 5093908 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180400 | (1 of 12) IPR011010//IPR013762 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCTAGCTCGTCGAACGTGAGTAGCTCCCGCTGGCGGCGGCGCGGCAC |
| Internal bar code: | GTGCTACTCGACGCTTACCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6665 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 122 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 122 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAGACCAGGAGACGATGT |
| Suggested primer 2: | GCCTTCATACTACGGCCTCC |