| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.010171 |
| Chromosome: | chromosome 3 |
| Location: | 5093911 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180400 | (1 of 12) IPR011010//IPR013762 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACATTTGTGGCCTTCCACTTGCTGGACCCAGCAGCCCCCCCGTCGCTGG |
| Internal bar code: | GATCATTACTCGTGTAGAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2371 |
| LEAP-Seq percent confirming: | 56.0976 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCATACTACGGCCTCC |
| Suggested primer 2: | TCGAGACCAGGAGACGATGT |