| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.010172 |
| Chromosome: | chromosome 13 |
| Location: | 3698799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588310 | MRPL47,uL29m,MRPL29 | Mitochondrial ribosomal protein L29; (1 of 1) K17428 - large subunit ribosomal protein L47 (MRPL47, NCM1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGCGCGATGAGGAGACCTGGCGGAGGGGATTGGGACAAGTGAGTGTC |
| Internal bar code: | GTTTATAGACGCTTCTCCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4871 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCCGCATGTAGCTGAC |
| Suggested primer 2: | GAAGCGGGGTTGTATGGTGA |