Insertion junction: CLIP2.010184_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.010184
Chromosome:chromosome 1
Location:6523080
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g046150 ICP55,XPA1 (1 of 2) 3.4.11.9 - Xaa-Pro aminopeptidase / X-Pro aminopeptidase; Intermediate Cleaving Peptidase 55 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTTTCGCGTTGCTGACAGCGAGTGGCAACCACAATCCCCGCCTCCGCA
Internal bar code:TCTCAGTTAAAAAGCTGGGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGCTGGAAAACGCAACA
Suggested primer 2:CTCACTCAACACTCCCCCAC