Insertion junction: CLIP2.010187_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010187
Chromosome:chromosome 16
Location:1973364
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g656400 SQD1 (1 of 1) 3.13.1.1 - UDP-sulfoquinovose synthase / Sulfite:UDP-glucose sulfotransferase; UDP-sulfoquinovose synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACTCGGCACCGCATACCAGCCCGCACGCCTTGTCCACGCATGCGCCGC
Internal bar code:GTTTGTTCGGTGGGATTCTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2033
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACTCCGACTTTGCTCCC
Suggested primer 2:GACCAATTGATCGTCGCACG