| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010209 |
| Chromosome: | chromosome 10 |
| Location: | 3969705 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446950 | (1 of 2) PTHR23181//PTHR23181:SF7 - RHO GTPASE ACTIVATING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGGACGCCACCGCCTGCGGCGTATAGCTCGTAAGCTGTGTAGGGCGT |
| Internal bar code: | TCGATCTTGCGGCATGCATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2160 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAGCCATCTGCCTTTGA |
| Suggested primer 2: | AGTTGCTCGCGAAGACTTCA |