Insertion junction: CLIP2.010210_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010210
Chromosome:chromosome 17
Location:2770268
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCCTGAATGAGCGCGCCTTCCTCGTTGGAGAAGAACTCGCCGCACACC
Internal bar code:CTGGTCTCCTTTTCTATGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:241
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTCCTTCTCCTCCTCCC
Suggested primer 2:GCTGGTGGTGTTGTAGAGCT