| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010214 |
| Chromosome: | chromosome 9 |
| Location: | 2993068 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393432 | (1 of 1) IPR002421//IPR029060 - 5'-3' exonuclease, N-terminal // PIN domain-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGCGGCGGAGGCTGCATGCATCGCGTCATGCGGGTGCAACGGCAGT |
| Internal bar code: | CGATGTTACTACGCAGTTCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1554 |
| LEAP-Seq percent confirming: | 86.1111 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGACGATGGAGAGCTGG |
| Suggested primer 2: | AGCAGCACTTGACATGGGAA |