Insertion junction: CLIP2.010219_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.010219
Chromosome:chromosome 17
Location:1561068
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g707137 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGGCTGCGACAAGTGTGGCAGCAGTAGCGCTTGCGCATGCGCTGGTA
Internal bar code:GCACCTATCAACTGTCTCAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:89
LEAP-Seq percent confirming:2.12766
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:AAAAAGCGATGCGTGTCCAC
Suggested primer 2:GATATGCGGTGATGGAGGCA