| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010268 |
| Chromosome: | chromosome 4 |
| Location: | 4071368 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233202 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAGCGCTGGTACGGCACGGCCGGGGCGTGCCAGAGCCTAAGGTGGCG |
| Internal bar code: | GCCTCAGTAGATTGACGGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTACGAGCCTACCACGC |
| Suggested primer 2: | GGCTTGGAAGATGAGGGCTT |