| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.010271 |
| Chromosome: | chromosome 7 |
| Location: | 1665064 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325450 | (1 of 4) PF06966 - Protein of unknown function (DUF1295) (DUF1295) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCTAGAATTAAGGGTTGACGCCCAGCAGTTGCTTAATGTTATTGTG |
| Internal bar code: | ATCGAAGTGAGCAATCGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCGTCCCATCCCATGACT |
| Suggested primer 2: | GTTAGGCCTTAAGCATGCGC |