Insertion junction: CLIP2.010272_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.010272
Chromosome:chromosome 3
Location:280276
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144404 (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) 3'UTR
Cre03.g144424 (1 of 5) PTHR32467//PTHR32467:SF15 - FAMILY NOT NAMED // AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMZ-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGATGAGCGCCGGTGGGACCCGCTGTGGCCTCCAGGCGCGAGTCACTG
Internal bar code:TGCGCCGTAACTCACCTAGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CACATCAGCAACAACACGCA
Suggested primer 2:TGCTGCCACTCCATAGCTTC