Insertion junction: CLIP2.010279_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.010279
Chromosome:chromosome 11
Location:3426629
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477250 (1 of 1) PF10187 - N-terminal domain of NEFA-interacting nuclear protein NIP30 (Nefa_Nip30_N) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACGTAACTAGTGCATCACGCACCACGCAACACATCCCGTATACGGTA
Internal bar code:GTCGAAAGCGCCAGATAAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1946
LEAP-Seq percent confirming:93.5484
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGGGACAAACTCGGACTC
Suggested primer 2:AGCCCCGATTACCTCAGTCT