| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010283 |
| Chromosome: | chromosome 1 |
| Location: | 1366405 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGAGCTACAGCCGCCGGCGCGGCAGCGCAGCCGCTCGTTCCGACTGC |
| Internal bar code: | AATCTTTGTTAGGGCAAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2224 |
| LEAP-Seq percent confirming: | 91.1392 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGGTCTGGTTGCAGAG |
| Suggested primer 2: | GCTATCACAGGCTCGACTCC |