Insertion junction: CLIP2.010291_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.010291
Chromosome:chromosome 13
Location:1136378
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569550 (1 of 17) PF08016 - Polycystin cation channel (PKD_channel) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATGAGCGAGGACGGCGCCGGCGGCGGCGACGGCGCCGAGCATGACCC
Internal bar code:TTGTGCTATTGTTACCTAAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:721
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCGTAGATGGACAGGTGG
Suggested primer 2:TCGTGCTGGTGTCCTTCTTC