| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010295 |
| Chromosome: | chromosome 2 |
| Location: | 5286799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112750 | (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTGCCTGCCCACATAACCCCTCAGCCCCTGAGCCCTTGCCCCCCTCC |
| Internal bar code: | CAGCTTTCCCGCTGACATGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1774 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGGTTCAGCTCGGTCAG |
| Suggested primer 2: | GCTTCACATTTCCCGCCATC |