| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.010313 |
| Chromosome: | chromosome 8 |
| Location: | 3731082 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380550 | SDR14 | (1 of 2) IPR002347//IPR013968 - Glucose/ribitol dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGTGCGCAGGTCCACGCCCCTGAAGGGGTGGTGAGGCGTGTGTGTGT |
| Internal bar code: | CGGTCCCAGCGCATTGGCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2000 |
| LEAP-Seq percent confirming: | 88.3117 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGCGGGACCTAACCAAC |
| Suggested primer 2: | TGTAAATGCCAGCCCGAACT |