| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010339 |
| Chromosome: | chromosome 10 |
| Location: | 2121022 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433150 | (1 of 1) PF01344//PF13415 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGGGGGCTGTCAAGCGCTGGGTCTTTGTCAACGTCGCCGGCGGCGG |
| Internal bar code: | CCCTTGGGAACCCCGGTGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTCAGGCATTGCAGGC |
| Suggested primer 2: | GCATGTCTGGCAATACAGCG |