| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010347 |
| Chromosome: | chromosome 12 |
| Location: | 1800039 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512750 | TEF20 | Notchless-like protein; (1 of 1) PTHR22847//PTHR22847:SF50 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCATGCATCCCGCTGCCTGCATGCGCCGTCGCACTGCAGCCCCACCG |
| Internal bar code: | CGCATCGGATATCAAGTCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1038 |
| LEAP-Seq percent confirming: | 5.88235 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAAAACGAAAGCAGGCG |
| Suggested primer 2: | AAAGCCACCTGTCCACAACA |