Insertion junction: CLIP2.010398_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010398
Chromosome:chromosome 2
Location:5564012
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g115300 AOT6 Amino acid transporter; (1 of 1) PTHR22950:SF72 - SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 6-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCCATCGTCCAGGGGCTGTCGCAGATGCTCCATGCTCCAATCATCTG
Internal bar code:GTATACACTTAATTTTACGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1111
LEAP-Seq percent confirming:23.8095
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GTAATGGTGGGGAGGGGTTG
Suggested primer 2:CAAGCCAGTCAACGCTCAAC