| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.010412 |
| Chromosome: | chromosome 10 |
| Location: | 796477 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGATTTGCCGCGGCCCCCGTGCACTGCCGGTAAGGGACGCGATGGGGA |
| Internal bar code: | GGGCAGTAGGCAGGCGCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2150 |
| LEAP-Seq percent confirming: | 89.8876 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGTTGCCAAGCAGCATG |
| Suggested primer 2: | ACTGTACCGACGAGTTGCTG |