Insertion junction: CLIP2.010414_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.010414
Chromosome:chromosome 17
Location:2771274
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGATGCAGACCTGGGCGCCGTCGTTGCCCAGCCCCAGACCCAGCTGCG
Internal bar code:TCCACCAATCGAGGATAACAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1351
LEAP-Seq percent confirming:26.3158
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCGCAGCTACTAGACGGC
Suggested primer 2:TTGTTCTCGGGGTCAACCAG