Insertion junction: CLIP2.010426_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010426
Chromosome:chromosome 6
Location:5505489
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g286500 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCGCCAACGGCGCCGCCGCCGCGCCCGCCGCCGCCCACCACGGCGC
Internal bar code:AGGGAACTTAGGGTCTATACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2032
LEAP-Seq percent confirming:52.6316
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACGGAGGAGGAGATTGC
Suggested primer 2:TTCCCATCATGCCACTCCAC