| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.010435 |
| Chromosome: | chromosome 2 |
| Location: | 5833740 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117250 | (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase | 3'UTR | |
| Cre02.g800244 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGGGGGCCCCATGCATGGGGGCTCCATGCCATCTCGCAATGGGATG |
| Internal bar code: | TGTATAGCACGGGTTGGAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1826 |
| LEAP-Seq percent confirming: | 77.0115 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGACCTTCGCAGGGAGAT |
| Suggested primer 2: | CTGTGCGATGTGCTATGTGC |