| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010442 |
| Chromosome: | chromosome 3 |
| Location: | 5437157 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184000 | 3'UTR | ||
| Cre03.g800346 | (1 of 10) PTHR11439//PTHR11439:SF185 - GAG-POL-RELATED RETROTRANSPOSON // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCTGGCCGCGTTACCAAAGGTTTTGCGGTACGTCGCCCTGCAGTCCA |
| Internal bar code: | AGGCTCGGGCAGACTCCGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1212 |
| LEAP-Seq percent confirming: | 56.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCACGTGCCCATTTTC |
| Suggested primer 2: | TGCTTTGTTAACCACACGCG |