| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010449 |
| Chromosome: | chromosome 7 |
| Location: | 2620410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330300 | (1 of 2) K14411 - RNA-binding protein Musashi (MSI) | CDS | |
| lncRNA_TCONS_00074821 | CDS | ||
| lncRNA_TCONS_00075115 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCTTCAACCTGCGGCAGCGAGCGAGCGCGGTGAGTGGAAGCTCGTGG |
| Internal bar code: | GCTACAGTTGATTTCCTCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGAACGCCCAGTGAGTT |
| Suggested primer 2: | CATTGGGTAGCTGCAGACCA |