Insertion junction: CLIP2.010466_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010466
Chromosome:chromosome 6
Location:8373981
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g307750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCACACACACCCTCGTGTGTCGACCCTTCCCCCTTGTGCGTTCCTGAC
Internal bar code:ATGATTAACAGGTGTGTTATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1225
LEAP-Seq percent confirming:34.1463
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:TCACCACATGGAGGTTCACG
Suggested primer 2:GTTAATGCGCGCACCTGTAG