| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010469 |
| Chromosome: | chromosome 1 |
| Location: | 4219997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028450 | (1 of 1) PTHR30544//PTHR30544:SF2 - 23S RRNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTCCTCCAACCTCACAGCCCCGCCCCGCCAGGCCCAGCCCGCCGCGT |
| Internal bar code: | GAATTCTCTCAACGAGGGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2367 |
| LEAP-Seq percent confirming: | 99.2188 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCTCTGCTTTCACCTT |
| Suggested primer 2: | ACCAGCTGCTCCACAATCTC |