| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.010474 |
| Chromosome: | chromosome 3 |
| Location: | 5520157 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184950 | MAW3 | Membrane-associated hydroxyproline-rich glycoprotein 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCTGGCGCCATCGCCAGCCTGCCGGCTTCTTCCTACCCCGACGGCCT |
| Internal bar code: | ATGACCGTACCCTAATCCCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1631 |
| LEAP-Seq percent confirming: | 75.7576 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGCCAGTCCTCAGTATC |
| Suggested primer 2: | AGGGCGGAACTGGATGAAAG |