| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.010478 |
| Chromosome: | chromosome 11 |
| Location: | 222871 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467554 | (1 of 15) PF01694 - Rhomboid family (Rhomboid) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAGCAATGACAGCCTTGACAGCATGGACCTTGACGGTGCGTGGCTG |
| Internal bar code: | CATCTTCCTTACTGGCGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 26.4706 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGAGTGGGGAGGTGGAT |
| Suggested primer 2: | CCTGATAACGACGACCGGAG |