Insertion junction: CLIP2.010490_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010490
Chromosome:chromosome 10
Location:1737188
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430200 ARS3 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCTGACCACGCGCCCCGACGACCAGAGCGACACGTGGATGCCTTCAC
Internal bar code:AGATAGGTCCCAGGAATTTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3339
LEAP-Seq percent confirming:22.2222
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGGCTAGCAGGGATAGC
Suggested primer 2:CAACAAACAGGACCAGCTGC