Insertion junction: CLIP2.010507_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010507
Chromosome:chromosome 6
Location:6128859
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291450 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGTGCACACCGACGGTGCGGGAGATGACGACGACCGCCCCTCGCACA
Internal bar code:TTGATCCGTTTGCCTTAGTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2414
LEAP-Seq percent confirming:91.8367
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAAAGGTCTGCTGGTACA
Suggested primer 2:CAGCAGTGAAAACGAGGTGC