| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010521 |
| Chromosome: | chromosome 10 |
| Location: | 2469964 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435750 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACATTCATGGGCAGGAGGGAACGCACCCCATCTCACATGCACACGCA |
| Internal bar code: | ACTAGGAAAAGCAAAGCTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1279 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGGATGCACACACACAC |
| Suggested primer 2: | GACCAGCTCCTGTTCGTTGA |