Insertion junction: CLIP2.010525_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010525
Chromosome:chromosome 7
Location:3938504
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g339550 FBB13 Flagellar/basal body protein 13 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCAGTGAACAAGCACCAGCCCGCTTTTGACGTTAGATGCTGGCAGAC
Internal bar code:TACAGTCTCACGACTGGTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1432
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CCATATTCGCACACGCACTG
Suggested primer 2:TGCTACTGCTGTGACACAGG