| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.010533 |
| Chromosome: | chromosome 17 |
| Location: | 1134454 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGGCTGGGCGCTACCGATGGGTCGCTGTTGGCGACCACCAGTCGGGG |
| Internal bar code: | GTACGTTTGTGTAGCGTCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 1.69492 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGACACCAGGCAGTTGC |
| Suggested primer 2: | TCAGACACTGTAGCTGCTGC |