Insertion junction: CLIP2.010546_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010546
Chromosome:chromosome 7
Location:4924957
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346698 PHC75 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACAGCATCTCGGAGTGATACGGCATTCTTTTGCTATCCCAAGTCCGA
Internal bar code:GACGCATCGAACATAGAATCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2845
LEAP-Seq percent confirming:18.617
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:153
LEAP-Seq n unique pos:188

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGGTTCCCGCAACAAAC
Suggested primer 2:CGTAGATGCATGTCGCTTGC